Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Breast

Cell type

MDA-MB-453

Primary Tissue

Breast

Site of Extraction

Effusion, Pericardial

Tissue Diagnosis

Carcinoma

Sample

source_name

MDA-MB-453

cell line

MDA-MB-453

culture conditions

Attached

treatment

DMSO (vehicle) for 4 hours

target protein

AR

chip antibody

anti-AR

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction were performed as described in Reddy et al., Genome Research, 2009.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

46320154

Reads aligned (%)

96.1

Duplicates removed (%)

5.2

Number of peaks

53865 (qval < 1E-05)

hg19

Number of total reads

46320154

Reads aligned (%)

95.6

Duplicates removed (%)

6.1

Number of peaks

53669 (qval < 1E-05)