GSM4777147: MDA-MB-453 Attached Vehicle; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Breast
Cell type
MDA-MB-453
Primary Tissue
Breast
Site of Extraction
Effusion, Pericardial
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-453
cell line
MDA-MB-453
culture conditions
Attached
treatment
DMSO (vehicle) for 4 hours
target protein
AR
chip antibody
anti-AR
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction were performed as described in Reddy et al., Genome Research, 2009.