Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Breast
Cell type
MDA-MB-453
Primary Tissue
Breast
Site of Extraction
Effusion, Pericardial
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-453
cell line
MDA-MB-453
culture conditions
Attached
treatment
DMSO (vehicle) for 4 hours
target protein
AR
chip antibody
anti-AR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction were performed as described in Reddy et al., Genome Research, 2009.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
46320154
Reads aligned (%)
96.1
Duplicates removed (%)
5.2
Number of peaks
53865 (qval < 1E-05)

hg19

Number of total reads
46320154
Reads aligned (%)
95.6
Duplicates removed (%)
6.1
Number of peaks
53669 (qval < 1E-05)

Base call quality data from DBCLS SRA